{"text_format":"3","workbook":"xlsx","n_sheets":2,"sheets":["Sanger测序订购表","通用引物"],"not_rendered":{"images":2,"external_links":1,"defined_names":3,"comments":1},"read":"whole","date_system":"1900"}

{"sheet":"Sanger测序订购表","index":0,"n_rows":27,"n_cols":14,"rows_kept":27,"merged":[[0,0,0,13],[1,1,1,3],[1,4,1,5],[1,6,1,13],[2,1,2,3],[2,4,2,5],[2,6,2,13],[3,1,3,3],[3,5,3,7],[3,9,3,13],[4,0,4,7],[4,8,4,13],[5,0,8,7],[5,8,8,13],[20,0,20,13],[21,0,21,13],[22,0,22,13],[23,0,23,13],[24,0,24,13],[25,0,25,13],[26,0,26,13]]}
["纳思生物-Sanger测序订购表"]
["订购日期:","年      月     日",null,null,"订购邮箱：",null,"nicerbio@wxnicer.com"]
["*姓名:",{"$nulls":3},"服务电话：",null,18021428048]
["*电话：",{"$nulls":3},"*E-mail：",{"$nulls":3},"*收货地址："]
["温馨提示：",{"$nulls":7},"样品无致病性风险证明"]
["1. 请务必在订单提交前仔细核对所填写的信息，此表格中*号的字段表示必填项,为不耽误您的测序进度，麻烦完整填写；\n2. 如果样品中如有高GC,Poly结构,发卡,ITR等，请您在特殊要求注明，以便我们选用不同的试剂和方法进行处理；\n3. 关于返样：菌液/平板样品，我们默认返还质粒，如需返还菌液，请备注告知；\n4. 样品和引物我们保存1个月，如需延长请及时告知；\n5. 如果您对测序结果有疑问，请及时联系本公司，如投诉时间超过 2个月，我们将有权不予受理。",{"$nulls":7},"本人寄送至纳思的样品承诺如下：\n1. 无任何致病性和传染性，或该样本已经过充分灭活，丧失了致病和传播能力。\n2. 所涉及的核酸序列，与新冠病毒COVID-19序列无任何相关性。\n3. 样品的传播与操作，在国家、地区及相关组织机构的允许范围内。 \n4. 无中国人类遗传资源材料或信息。"]
[]
[]
[]
["编号","*样品名称","*样品类型","片段长度     （PCR样品必填*）","载体名称","抗性（平板，菌液必填*）"," *引物名称","引物类型","引物序列（需纳思合成必填*）","自备引物浓度（uM）","特殊要求（高GC,poly,发卡,ITR）","测序要求（正向，反向，双向，测通）","是否返样","备注"]
[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
["送样标准："]
["①菌液：>50μL，注明抗性，免费提供Amp,Kan两种，其余抗性需自备并注明使用浓度，低拷贝建议直接送质粒。注：菌液抽提两次未提取成功会取消测序，抽提的质粒只满足测序要求。"]
["②质粒：>50ng/μL，>20μL（双蒸水溶），大片段质粒请注明载体长度（>10K)。"]
["③PCR已纯化：>10ng/μL，>20μL（双蒸水溶），条带单一，清晰可见，注明片段长度。"]
["④PCR未纯化：>10ng/μL，>10μL，如需切胶建议>50μL，并注明片段长度。注：＜200bp的样品建议克隆后再测序。"]
["⑤平板：菌斑清晰，无明显卫星菌落。"]
["⑥客户自行提供测序引物，建议浓度不低于5μM(pmol/µL)；同一条引物测1个反应，需至少提供5µl，若测多个反应，每个反应按2µL计算，例如：测10个反应，可提供20µL。"]

{"sheet":"通用引物","index":1,"n_rows":146,"n_cols":2,"rows_kept":146}
["引物名称","纳思生物测序通用引物序列(5'-3')"]
["M13F-77","GATGTGCTGCAAGGCGATTA"]
["M13R-88","TTATGCTTCCGGCTCGTATG"]
["M13F-47","CGCCAGGGTTTTCCCAGTCACGAC"]
["M13R-48","AGCGGATAACAATTTCACACAGGA"]
["M13F","TGTAAAACGACGGCCAGT"]
["M13R","CAGGAAACAGCTATGACC"]
["3-AOX      ","GCAAATGGCATTCTGACATCC"]
["5-AOX       ","GACTGGTTCCAATTGACAAG"]
["Pcold-R","GGGCCTGCGCATTCTCATTG"]
["Pcold-F","cagtgtagtaaggcaagtcc"]
["PcoldTF-F","CCACTTTCAACGAGCTGATG"]
["GLp2   ","CTTTATGTTTTTGGCGTCTTCC"]
["RVp3   ","CTAGCAAAATAGGCTGTCCC"]
["RVp4","GACGATAGTCATGCCCCGCG"]
["PBV220F","AAGAAGGGCAGCATTCAAAG"]
["PBV220R","CTGCGTTCTGATTTAATCTG"]
["PFastBac-F","aaatgataaccatctcgc"]
["PFastBac-R","TTCAGGTTCAGGGGGAGGTG"]
["PFastBAC-HT-F","TTCATACCGTCCCACCATCG"]
["PET32a-seqR","CTGGTTCTGGCCATATGCAC"]
["PET43.1a-seqR","ACACTACCATCGGCGCTACG"]
["PJET1.2-seqR","TATACAGCCTGAAAATCTTG"]
["PJET1.2-seqF","TGTGCCTGAACACCATATCC"]
["PTGE5-seqF","CCGATCTGGCCATACACTTG"]
["PTGE5-seqR","CATATGTCCTTCCGAGTGAG"]
["U6","GGACTATCATATGCTTACCG"]
["U6-5","TACGATACAAGGCTGTTAGAGAG"]
["M13-96","CCCTCATAGTTAGCGTAACG"]
["M13F-75","GATGCCGGGAGCAGACAAGC"]
["M13R-76","GTGAGCGAGGAAGCGGAAGA"]
["siRNA-5'","cctccgatcgttgtcagaag"]
["Amp-F","ATAATACCGCGCCACATAGC"]
["pET-seqF","GGCCCCAAGGGGTTATGCTAGT"]
["pET-seqR","GATCCCGCGAAATTAATACG"]
["pENTR4-seqF","AGCAGAAGGCCATCCTGACG"]
["pENTR4-seqR","GTGCAATGTAACATCAGAGA"]
["pQE30-seqF","TGAGCGGATAACAATTTCAC"]
["pQE30-seqR","CAACCGAGCGTTCTGAACAA"]
["puc57-1.8k-SeqF ","GAAGCATTTATCAGGGTTAT"]
["puc57-1.8k-SeqR ","GGCGGAGCCTATGGAAAAAC"]
["pGEX-5'","GGGCTGGCAAGCCACGTTTGGTG"]
["pGEX-3'","CCGGGAGCTGCATGTGTCAGAGG"]
["pCMV-F","TCTAAAAGCTGCGGAATTGT"]
["pCMV-R ","TCCAAACTCATCAATGTATC"]
["T3","ATTAACCCTCACTAAAGGGA"]
["T7","TAATACGACTCACTATAGGG"]
["T7T","TGCTAGTTATTGCTCAGCGG"]
["3-AD ","AGATGGTGCACGATGCACAG"]
["3-BD","TAAGAGTCACTTTAAAATTTGTATAC"]
["GAL4AD/5-AD","TACCACTACAATGGATG"]
["GAL4-BD","TCATCGGAAGAGAGTAG"]
["pEGFP-C-5","CATGGTCCTGCTGGAGTTCGTG"]
["pEGFP-C-3","TATGGCTGATTATGATCAGT"]
["S-tag","GAACGCCAGCACATGGAC"]
["PYES2-SEQF","TCTCAAGCAAGGTTTTCAGT"]
["PYES2-SEQR","CGGTTTGTATTACTTCTTATTC"]
["MalE","GGTCGTCAGACTGTCGATGAAGCC"]
["CYC1-Terminator","GTGACATAACTAATTACATGATG"]
["pMAL-C2X-F","TGCGTACTGCGGTGATCAAC"]
["pMAL-C2X-R","CTGCAAGGCGATTAAGTTGG"]
["pMal-C5X-F","CCAGAAAGGTGAAATCATGC"]
["pMal-C5X-R","GATTTGTCCTACTCAGGAGA"]
["35S","GACGCACAATCCCACTATCC"]
["NOS-R","AATCATCGCAAGACCGGC"]
["pDONR207-F","GGCGGATTTGTCCTACTCAG"]
["pDONR207-R","CTCGACGTTTCCCGTTGAAT"]
["Up1","GATCTCGACGCTCTCCCT"]
["DOWN1","GATTATGCGGCCGTGTACAA"]
["DuetUP2","TTGTACACGGCCGCATAATC"]
["LK01-5 Primer","agggatggttggttggtggg"]
["EF1a-F","TCAAGCCTCAGACAGTGGTTC"]
["CMV-30","ATAACCCCGCCCCGTTG"]
["CMV-24","TTAGGACAAGGCTGGTGG"]
["SP6 ","ATTTAGGTGACACTATAG"]
["CMV-F","GGGCGGTAGGCGTGTACGGTGG"]
["CMV-R","GTTCACGGTGCCCTCC"]
["a-factor      ","TACTATTGCCAGCATTGCTGC"]
["pFlag-CMV-F","GGTAGGCGTGTACGGTGG"]
["pFlag-CMV-R","GCACTGGAGTGGCAACTT"]
["pIRES2-EGFP-SEQF","ATGCCCGAAGGCTACGTCCA"]
["pIRES2-EGFP-SEQR","GACGTTGTGGCTGTTGTAGT"]
["PEGFP-N-3  ","CGTCGCCGTCCAGCTCGACCAG"]
["PEGFP-N-5 ","TGGGAGGTCTATATAAGCAGAG"]
["PCDNA3.1R","ACAGTGGGAGTGGCACCTTC"]
["PCDNA3.1F","CTAGAGAACCCACTGCTTAC"]
["Psumo-F","GGCCCCTGAAGATTTGGACA"]
["Sv40-PRO-F","TATTTATGCAGAGGCCGAGG"]
["Sv40-PA-R","GAAATTTGTGATGCTATTGC"]
["BGH","TAGAAGGCACAGTCGAGG"]
["H1.3F","GACGTCAATGGGAGTTTGTT"]
["PCAGGS-SEQF","TTCGGCTTCTGGCGTGTGAC"]
["PCAGGS-SEQR","TATGTCCTTCCGAGTGAGAG"]
["27F","AGAGTTTGATCCTGGCTCAG"]
["1492R","TACGGCTACCTTGTACGACTT"]
["1525R","AAGGAGGTGATCCAACC"]
["NS1","GTAGTCATATGCTTGTCTC"]
["NS4","CTTCCGTCAATTCCTTTAAG"]
["NS8","TCCGCAGGTTCACCTACGGA"]
["ITS1","TCCGTAGGTGAACCTGCGG"]
["ITS4","TCCTCCGCTTATTGATATGC"]
["ITS5","GGAAGTAAAAGTCGTAACAAGG"]
["NL1","GCATATCAATAAGCGGAGGAAAAG"]
["NL4","GGTCCGTGTTTCAAGACGG"]
["pGL3-Basic-SeqF","TTATTTTCATTACATCTGTG"]
["pGL3-Basic-SeqR","CTTCATAGCCTTATGCAGTT"]
["pBAD-F","ATGCCATAGCATTTTTATCC"]
["pBAD-R","GATTTAATCTGTATCAGG"]
["PMIRGLO-SEQF","GCGCGGTGGTGTTGTGTTCG"]
["PMIRGLO-SEQR","TGTGGTTTGTCCAAACTCA"]
["PCB2004-F","AGAACACGGGGGACTCTA"]
["PCB2004-R","ATCGCAAGACCGGCAACA"]
["PSICHECK2-F","TCCACTTCAGCCAGGAGGAC"]
["PSICHECK2-R","GCGAGGTCCGAAGACTCATT"]
["PGWB5-F","GGAGAGAACACGGGGGACTC"]
["PGWB5-R","GGAAATTCGAGCTCGGGCCGC"]
["PBABE-5","CTTTATCCAGCCCTCAC"]
["PBABE-3","ACCCTAACTGACACACATTCC"]
["PCDH-R","CACGACATCACTTTCCCAGT"]
["T7-sg-CX-F","TGCAAGGCGATTAAGTTGGG"]
["PBI121-SEQF","GCAAGTGGATTGATGTGAT"]
["PBI121-SEQR","CGCATAATTACGAATATCTG"]
["PX330-F","GCATATACGATACAAGGCTG"]
["PX330-R","GCTCACCTCGACCATGGTAA"]
["PCAG-5","CGTGTGACCGGCGGCTCTAGAGCCTC"]
["PCAG-F","GCAACGTGCTGGTTATTGTG"]
["PCAM1301-NCOI-R","TTTACACCATAACCAATCAA"]
["mCherry-F","CCCCGTAATGCAGAAGAAGA"]
["EGFP-C","AGCACCCAGTCCGCCCTGAGC"]
["PTT5-SEQF","TGGCCATACACTTGAGTGAC"]
["PTT5-SEQR","CCATATGTCCTTCCGAGTGA"]
["H1 promoter","TCGCTATGTGTTCTGGGAAA"]
["mCherry-R","TTGGTCACCTTCAGCTTGG"]
["WPRE-R","CATAGCGTAAAAGGAGCAACA"]
["PGR107-SEQF","GGAATCAATCACAGTGTTGG"]
["PGR107-SEQR","TTGAGGTAGTTGACCCTATG"]
["pCMV5-Fwd ","CGCAAATGGGCGGTAGGCGTG"]
["pCMV5-Rev","CCTCCACCCCATAATATTATAGAAGGACAC"]
["pSPYC(N)E-F","GACGTAAGGGATGACGCAC"]
["PSPYNE-R","CGCCCTTGCTCACCATAA"]
["PSPYCE-R","GATGCCGTTCTTCTGCTTGT"]
["1541R","AAGGAGGTGATCCAGCC"]
["1495R","CTACGGCTACCTTGTTACGA "]
["EF1a-R","GAGCCAGTACACGACATCAC"]
["M13F-55","CGGGCCTCTTCGCTATTA"]
["M13R-66","GCGCGTTGGCCGATTCAT"]